Clinical trial • Phase III • Rare Disease

RECOMBINANT HUMAN ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE 1 FUSED TO THE FC FRAGMENT OF IGG1 for Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) deficiency

Phase III trial of RECOMBINANT HUMAN ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE 1 FUSED TO THE FC FRAGMENT OF IGG1 for Ectonucleotide pyrophosphatas…

Overview

Trial Therapeutic Area
Rare Disease
Trial Disease
Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) deficiency
Trial Stage
Phase III
Drug Modality
Peptide/protein/enzyme
Paediatric Trial
Yes
Orphan Drug
Yes

Key dates

Initial CTIS Submission Date
03-11-2023
First CTIS Authorization Date
06-03-2024

Trial design

Randomised, open-label, active control: conventional therapy provided locally by each site (active control); no specific drug name, dose, or standardized schedule specified in part i, adaptive Phase III trial in France, Spain.

Randomised
Yes
Open Label
Yes
Comparator
Active control: Conventional therapy provided locally by each site (active control); no specific drug name, dose, or standardized schedule specified in Part I
Adaptive
True, dose-escalation and extension elements are planned: initial Randomized Treatment Period (RTP) with INZ-701 2.4 mg/kg once weekly vs active control; Low Dose Open-Label Extension where all participants receive INZ-701 2.4 mg/kg QW while primary analysis is pending; if Dose Escalation Criteria are met, participants may enter a Randomized Extension Period and then High-Dose Open-Label Extension with higher frequency dosing (e.g. INZ-701 1.8 mg/kg twice weekly).
Single Multiple Or Escalation Dose Combined
Yes
Target Sample Size
26
Trial Duration For Participant
364

Eligibility

Recruits 26 paediatric patients.

Pregnancy Exclusion
Women of childbearing potential (WOCBP, as defined in Clinical Trials Coordination Group [CTCG 2024]) must have a negative serum pregnancy test at Screening and must not be breastfeeding
Vulnerable Population
Participants are children (males and females ≥1 year and <13 years). Caregiver’s written informed consent is required prior to any research procedures and study participant assent is required in accordance with local regulations. Age-specific assent and consent materials are provided (e.g. assent forms for ages 4-6 and 7-12, parent/legal guardian ICF). eConsent (ClinOne) and telephone pre-ICF consent procedures are available; materials and ICF/assent documents are provided in multiple languages (English, Spanish, French, Italian, Portuguese, Polish, German/DE-AT among others) per submitted documentation.

Inclusion criteria

  • {"criterion_text":"- Caregiver’s written informed consent after the nature of the study has been explained, and prior to any research-related procedures, per International Conference on Harmonisation (ICH) Good Clinical Practice (GCP)"}
  • {"criterion_text":"- Males who are sexually active must agree to use condoms from the period following first dose of INZ-701 through 30 days after the last dose of INZ-701"}
  • {"criterion_text":"- WOCBP and partners of fertile males who are WOCBP must be using or must agree to use a highly effective form of contraception (as per CTCG) from at least 1 month before the first dose of INZ-701 through 30 days after the last dose of INZ-701 (greater than 5 half-lives of INZ-701)"}
  • {"criterion_text":"- In the opinion of the Investigator, able to complete all aspects of the study"}
  • {"criterion_text":"- Study participant’s assent in accordance with local regulations"}
  • {"criterion_text":"- A confirmed postnatal molecular genetic diagnosis of ENPP1 Deficiency with biallelic mutations (ie, homozygous or compound heterozygous) performed by a College of American Pathologists/Clinical Laboratory Improvement Amendments (CAP/CLIA) certified laboratory or regional equivalent"}
  • {"criterion_text":"- Males and females ≥1 year and <13 years of age at Study Day 1"}
  • {"criterion_text":"- Open growth plates of the distal femur and proximal tibia in both legs"}
  • {"criterion_text":"- Plasma PPi concentration of <1400 nM at Screening"}
  • {"criterion_text":"- 25(OH)D levels of ≥12 ng/mL at Screening"}
  • {"criterion_text":"- Radiographic evidence of skeletal abnormalities based on an RSS ≥2"}
  • {"criterion_text":"- Women of childbearing potential (WOCBP, as defined in Clinical Trials Coordination Group [CTCG 2024]) must have a negative serum pregnancy test at Screening and must not be breastfeeding"}

Exclusion criteria

  • {"criterion_text":"- In the opinion of the Investigator, has clinically significant disease or laboratory abnormality not associated with ENPP1 Deficiency that will preclude study participation and/or may confound the interpretation of study results"}
  • {"criterion_text":"- If receiving any of the following prohibited medications as indicated in the protocol: systemic corticosteroids (>5 mg prednisone equivalent per day), anti-FGF23, and oral and/or IV bisphosphonates"}
  • {"criterion_text":"- Unable or unwilling to discontinue calcitriol or other active forms of vitamin D3 (or analogs) within 7 days prior to Study Day 1 and/or oral phosphate supplements within 36 hours prior to Study Day 1 if randomized to the INZ-701 arm"}
  • {"criterion_text":"- Planned orthopedic surgery or other procedures that may confound the interpretation of study results during the 52-week RTP"}
  • {"criterion_text":"- Known intolerance to INZ-701 or any of its excipients"}
  • {"criterion_text":"- A positive COVID-19 test within 5 days prior to Randomization, only if required as per local regulations or institutional policy"}
  • {"criterion_text":"- Previous treatment with INZ-701"}
  • {"criterion_text":"- Concurrent participation in another interventional clinical study and/or has received an investigational drug within 5 half-lives of the last dose or within 4 weeks prior to the first dose of INZ-701, whichever is longer, or use of an investigational device"}

Endpoints

Primary endpoints

  • {"endpoint_text":"- Change from Baseline in plasma PPi concentration through Week 52","definition_or_measurement_approach":"Plasma PPi concentration measured and change from baseline assessed through Week 52 (to determine if INZ-701 increases PPi levels)"}
  • {"endpoint_text":"- RGI-C global score through Week 52","definition_or_measurement_approach":"Change from baseline in Radiographic Global Impression of Change (RGI-C) global score through Week 52 (to assess improvement in skeletal abnormalities)"}

Secondary endpoints

  • {"endpoint_text":"- Change from Baseline in RSS total score through Week 52","definition_or_measurement_approach":"Change from baseline in Rickets Severity Score (RSS) total score through Week 52"}
  • {"endpoint_text":"- Change from Baseline in growth Z-score (height/body length and weight) through Week 52","definition_or_measurement_approach":"Change from baseline in growth Z-scores for height/body length and weight through Week 52"}
  • {"endpoint_text":"- Measurement of INZ-701 serum concentration and enzyme activity","definition_or_measurement_approach":"Pharmacokinetic measurement of INZ-701 serum concentration and assessment of ENPP1 enzyme activity"}
  • {"endpoint_text":"- Please refer to the protocol for Tertiary and Safety endpoints","definition_or_measurement_approach":"Details of tertiary and safety endpoints are provided in the study protocol"}

Recruitment

Registry Or Advocacy Recruitment
True, Rare Disease Research Partners Limited
Digital Remote Recruitment
True, includes eConsent (ClinOne), electronic patient diary (Umotif), remote pre-ICF telephone consent and other digital patient-facing materials
Planned Sample Size
26
Recruitment Window Months
34
Consent Approach
Caregiver/parental written informed consent required prior to any research procedures; study participant assent required in accordance with local regulations. eConsent is implemented (ClinOne) with patient-facing submission packets and reference manuals. Age-specific assent and information sheets are provided (assent 4-6 years; assent 7-12 years) and parent/legal guardian ICFs are provided. Documents and consent materials are available in multiple languages (English, Spanish, French, Italian, Portuguese, Polish, German/DE-AT, etc.) as per submitted documentation.

Methods

  • Family webinars (recruitment material titled 'Family Webinar' available in multiple languages)
  • Informational booklets for families (available in multiple languages)
  • Scout Travel Considerations Questionnaire (documents to assess travel needs and support for participants/families)
  • Scout pre-ICF telephone data consent (telephone contact for pre-ICF screening and data consent)
  • E-consent via ClinOne (electronic consent platform and patient reference manuals)
  • Patient-facing materials including Subject ID cards and Notices regarding travel insurance

Geography

Total Number Of Sites
2
Total Number Of Participants
8

France

Earliest CTIS Part Ii Submission Date
14-12-2023
Latest Decision Or Authorization Date
30-03-2026
Processing Time Days
837
Number Of Sites
1
Number Of Participants
1

Sites

Site Name
Assistance Publique Hopitaux De Paris
Department Name
Pediatric Endocrinology and Diabetes for Children
Principal Investigator Name
Agnès LINGLART
Principal Investigator Email
agnes.linglart@aphp.fr
Contact Person Name
Agnès LINGLART
Contact Person Email
agnes.linglart@aphp.fr
Number Of Participants
1

Spain

Earliest CTIS Part Ii Submission Date
26-02-2024
Latest Decision Or Authorization Date
08-04-2025
Processing Time Days
407
Number Of Sites
1
Number Of Participants
7

Sites

Site Name
Sant Joan De Deu Barcelona Hospital
Department Name
Pediatric
Principal Investigator Name
Pedro Arango Sancho
Principal Investigator Email
pedro.arango@sjd.es
Contact Person Name
Pedro Arango Sancho
Contact Person Email
pedro.arango@sjd.es
Number Of Participants
7

Sponsor

Primary sponsor

Full Name
Inozyme Pharma Inc.
Organisation Type
Pharmaceutical company
Country Of Registered Address
United States

Contract research organisations

Name
Worldwide Clinical Trials
Responsibilities
Site agreement; operational support; sponsorDuties include codes 1,12,15,5 (as listed)
Name
Bioclinica Inc.
Responsibilities
X-Ray central collection and reader
Name
Clinone Inc.
Responsibilities
E-consent implementation
Name
Medrio Inc.
Responsibilities
Electronic data capture / data management (sponsorDuties codes 3 and 7 listed)
Name
Red Nucleus Solutions LLC
Responsibilities
Video support on scales

Third parties

  • {"country":"Germany","full_name":"Catalent Germany Schorndorf GmbH","duties_or_roles":"Depot/Clinical Supplies","organisation_type":"Pharmaceutical company"}
  • {"country":"United States","full_name":"Alliance Pharma Inc.","duties_or_roles":"Analyzing samples for PPi, PK, ENPP1 Activity, Immunogenicity","organisation_type":"Pharmaceutical company"}
  • {"country":"United States","full_name":"Biomarin Pharmaceutical Inc.","duties_or_roles":"","organisation_type":"Pharmaceutical company"}
  • {"country":"United States","full_name":"Labcorp Central Laboratory Services LP","duties_or_roles":"Biomarkers - CTX (for Type 1 Collagen Antigen)","organisation_type":"Pharmaceutical company"}
  • {"country":"United Kingdom","full_name":"Umotif Limited","duties_or_roles":"Electronic Diary","organisation_type":"Non-Pharmaceutical company"}
  • {"country":"United Kingdom","full_name":"Rare Disease Research Partners Limited","duties_or_roles":"Assist study participants and their families","organisation_type":"Patient organisation/association"}
  • {"country":"United Kingdom","full_name":"Mde Services Group Limited","duties_or_roles":"Nursing services; Patient facing materials and reimbursement","organisation_type":"Hospital/Clinic/Other health care facility"}
  • {"country":"Switzerland","full_name":"Labcorp Central Laboratory Services S.a.r.l.","duties_or_roles":"Serum Chemistry and Hematology, Urinalysis, Biomarkers (PINP & FGF23)","organisation_type":"Pharmaceutical company"}
  • {"country":"United States","full_name":"Bioclinica Inc.","duties_or_roles":"X-Ray central collection and reader","organisation_type":"Laboratory/Research/Testing facility"}
  • {"country":"Croatia","full_name":"Worldwide Clinical Trials","duties_or_roles":"Site agreement; multiple operational responsibilities (codes 1,12,15,5 as listed)","organisation_type":"Pharmaceutical company"}
  • {"country":"United States","full_name":"Clinone Inc.","duties_or_roles":"E-consent","organisation_type":"Pharmaceutical company"}
  • {"country":"United States","full_name":"Red Nucleus Solutions LLC","duties_or_roles":"Video support on scales","organisation_type":"Pharmaceutical company"}
  • {"country":"United States","full_name":"Medrio Inc.","duties_or_roles":"Responsibilities indicated by sponsor duty codes (3,7) as listed","organisation_type":"Pharmaceutical company"}

Investigational products

Investigational Product Name
INZ-701
Active Substance
RECOMBINANT HUMAN ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE 1 FUSED TO THE FC FRAGMENT OF IGG1
Modality
Peptide/protein/enzyme
Routes Of Administration
SUBCUTANEOUS USE
Route
Subcutaneous
Authorisation Status
Unapproved (investigational)
Orphan Designation
Yes
Starting Dose
2.4 mg/kg once weekly
Dose Levels
2.4 mg/kg once weekly; 1.8 mg/kg twice weekly (in Randomized Extension/High-Dose extension periods)
Frequency
Once weekly (RTP); Twice weekly (REP/high-dose extension)
Maximum Dose
2.4 mg/kg (product data field 'maxTotalDoseAmount' = 2.4 mg/kg)
Dose Escalation Increase
Initial: 2.4 mg/kg once weekly; Following (if dose escalation criteria met): 1.8 mg/kg twice weekly

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