Clinical trial • Phase III • Neurology

TENECTEPLASE for Ischaemic stroke|Large vessel occlusion stroke (LVOS)

Phase III trial of TENECTEPLASE for Ischaemic stroke|Large vessel occlusion stroke (LVOS).

Overview

Trial Therapeutic Area
Neurology
Trial Disease
Ischaemic stroke|Large vessel occlusion stroke (LVOS)
Trial Stage
Phase III
Drug Modality
Peptide/protein/enzyme

Key dates

Initial CTIS Submission Date
15-12-2025
First CTIS Authorization Date
12-03-2026

Trial design

Randomised, open-label, tenecteplase (metalyse 5 000 units (25 mg) powder for solution for injection; tenecteplase iv bolus, up to 25 mg) followed by mechanical thrombectomy (per standard protocol) versus mechanical thrombectomy alone.-controlled Phase III trial in France.

Randomised
Yes
Open Label
Yes
Comparator
Tenecteplase (Metalyse 5 000 units (25 mg) powder for solution for injection; tenecteplase IV bolus, up to 25 mg) followed by mechanical thrombectomy (per standard protocol) versus mechanical thrombectomy alone.
Target Sample Size
486
Trial Duration For Participant
365

Eligibility

Recruits 486 Vulnerable population flag set. Patients under legal protection (tutorship or curatorship) and patients deprived of freedom are excluded. Consent may be provided by the patient or by a trustworthy person / family member / close relative; inclusion in emergency is allowed with written informed consent required as soon as possible thereafter (per article 35 of EU regulation 536/2014). Dedicated consent documents exist for trusted person/relative (trusted person-relative ICF) and adult-specific ICFs are provided..

Pregnancy Exclusion
Pregnancy or breastfeeding woman
Vulnerable Population
Vulnerable population flag set. Patients under legal protection (tutorship or curatorship) and patients deprived of freedom are excluded. Consent may be provided by the patient or by a trustworthy person / family member / close relative; inclusion in emergency is allowed with written informed consent required as soon as possible thereafter (per article 35 of EU regulation 536/2014). Dedicated consent documents exist for trusted person/relative (trusted person-relative ICF) and adult-specific ICFs are provided.

Inclusion criteria

  • {"criterion_text":"- Age ≥ 18 years"}
  • {"criterion_text":"- mRS ≤ 1 before stroke"}
  • {"criterion_text":"- Anterior circulation LVOS eligible to MT within 24 hours of onset or unknown onset with a DWI-FLAIR mismatch"}
  • {"criterion_text":"- Large core defined either as: - ASPECTS 2-5 or a core volume between 70 and 130 ml on MRI or perfusion CT for patients with process times compatible with IVT administration within 4.5 hours of onset or unknown onset with process times compatible with IVT administration within 4.5 hours of last seen well or unknown onset with a DWI-FLAIR mismatch - ASPECTS 2- 5 with a core volume ≤ 70 ml and core/perfusion mismatch > 1.2 for patients with process times compatible with IVT administration within 4.5 and 9 hours of onset, defined as the mid-point between last known to be normal and symptoms constatation in case of unknown onset"}
  • {"criterion_text":"- Written informed consent signed by the patient or the trustworthy person / family member / close relative, or inclusion in case of emergency. (To note, in the latter case, written informed consent should be signed by the patient (if needed, by trustworthy person, family member or close relative) as soon as possible (article 35 of the European regulation N°536/2014))."}

Exclusion criteria

  • {"criterion_text":"- Anterior circulation stroke with a distal occlusion not eligible to MT"}
  • {"criterion_text":"- Posterior circulation stroke"}
  • {"criterion_text":"- Pregnancy or breastfeeding woman"}
  • {"criterion_text":"- Any contraindication to IVT other than those related to the NIHSS score upper limit, infarct size and symptoms-to-onset time, such as (but not limited to): * Persistent incapacity to lower blood pressure under 185/110 mmHg * Respiratory or hemodynamic failure * Externalized bleeding"}
  • {"criterion_text":"- Any contra-indication to MT: * Contra-indication to femoral, radial or humeral arterial puncture * Allergy to iodinated contrast media * Known Renal insufficiency at inclusion time (confirmed biologically by a creatinine clearance < 30 ml/min calculated with the Cockcroft-Gault formula)"}
  • {"criterion_text":"- Anticipated life expectancy of less than 3 months"}
  • {"criterion_text":"- Participation in another interventional clinical trial evaluating a health product or any randomized clinical trial"}
  • {"criterion_text":"- Absence of affiliation to National French social security system"}
  • {"criterion_text":"- Under legal protection measure (tutorship or curatorship) and patient deprived of freedom"}

Endpoints

Primary endpoints

  • {"endpoint_text":"- The primary outcome is the rate of good functional outcome (independent ambulation) at 3 months defined as a modified Rankin scale (mRS) score of 0-3. mRS scores will be determined by certified raters unaware of the treatment arm or baseline characteristics of the individual patient by in person interview or, if not possible, by telephone.","definition_or_measurement_approach":"Good functional outcome defined as mRS score 0-3 at 3 months; mRS determined by certified raters blinded to treatment and baseline characteristics by in-person interview or, if not possible, by telephone."}

Secondary endpoints

  • {"endpoint_text":"- Clinical outcomes -\tearly neurological improvement, defined as a ≥ 8-points decrease of the NIHSS score or a NIHSS score ≤ 1 at day 1 -\t3-month functional independence (mRS score ≤ 2) -\tdistribution of 3-month mRS scores -\tone-year independent ambulation (mRS score ≤ 3) -\tone-year functional independence (mRS score ≤ 2)","definition_or_measurement_approach":"Early neurological improvement defined as ≥8-point decrease in NIHSS or NIHSS ≤1 at day 1; 3-month and 1-year functional outcomes measured by mRS with specified thresholds; distribution of mRS at 3 months."}
  • {"endpoint_text":"- Radiological outcomes -\tsuccessful, excellent and complete recanalisation rates defined as an extended Treatment In Cerebral Ischemia (eTICI) scores of 2b50/2b67/2c/3, 2c/3 and 3 respectively on the first angiographic run, after the first pass and at the end of the procedure -\tmean change in infarct volume from baseline at day 1, defined as: (day 1 volume) – (baseline volume","definition_or_measurement_approach":"Recanalisation rates defined by eTICI categories (2b50/2b67/2c/3 etc.) measured on angiographic runs (first run, after first pass, end of procedure); mean change in infarct volume from baseline to day 1 calculated as (day 1 volume) – (baseline volume)."}
  • {"endpoint_text":"- Safety outcomes -\tearly neurological worsening defined as a ≥ 4-point increase on the NIHSS score within 24 hours due to the stroke itself -\tintracranial hemorrhage (ICH) according to the Heidelberg Bleeding Classification -\tsymptomatic ICH, scored according to the Heidelberg Bleeding Classification -\t3-month and 1-year mortality -\tadverse events -\tserious adverse events","definition_or_measurement_approach":"Early neurological worsening = ≥4-point increase on NIHSS within 24 hours attributable to stroke; ICH and symptomatic ICH adjudicated per Heidelberg Bleeding Classification; mortality at 3 months and 1 year; standard AE/SAE reporting."}
  • {"endpoint_text":"- Incremental cost-utility ratio","definition_or_measurement_approach":"Health economic outcome comparing incremental cost-utility between treatment strategies (details not further specified in CTIS JSON)."}

Recruitment

Planned Sample Size
486
Recruitment Window Months
36
Consent Approach
Written informed consent required from the patient or from a trustworthy person / family member / close relative. Emergency inclusion allowed with written informed consent to be obtained from the patient (or if needed from the trustworthy person/family/close relative) as soon as possible thereafter (per Article 35 of EU regulation 536/2014). Separate subject information and informed consent forms exist for adults and for trusted person/relative; no participant assent for minors (trial enrols adults ≥18). Languages of consent documents are not specified in the CTIS JSON.

Geography

Total Number Of Sites
36
Total Number Of Participants
486

France

Earliest CTIS Part Ii Submission Date
26-01-2026
Latest Decision Or Authorization Date
12-03-2026
Processing Time Days
45
Number Of Sites
36
Number Of Participants
486

Sites

Site Name
Centre Hospitalier Universitaire De Montpellier
Department Name
Neurology
Principal Investigator Name
Caroline ARQUIZAN
Principal Investigator Email
c-arquizan@chu-montpellier.fr
Contact Person Name
Caroline ARQUIZAN
Contact Person Email
c-arquizan@chu-montpellier.fr
Site Name
Centre Hospitalier Universitaire Grenoble Alpes
Department Name
Neurology
Principal Investigator Name
Olivier DETANTE
Principal Investigator Email
odetante@chu-grenoble.fr
Contact Person Name
Olivier DETANTE
Contact Person Email
odetante@chu-grenoble.fr
Site Name
Centre Hospitalier Regional Et Universitaire De Brest
Department Name
Neurology
Principal Investigator Name
Serge TIMSIT
Principal Investigator Email
serge.timsit@chu-brest.fr
Contact Person Name
Serge TIMSIT
Contact Person Email
serge.timsit@chu-brest.fr
Site Name
Centre Hospitalier Universitaire De Nantes
Department Name
Neurology
Principal Investigator Name
Benoit GUILLON
Principal Investigator Email
benoit.guillon@chu-nantes.fr
Contact Person Name
Benoit GUILLON
Contact Person Email
benoit.guillon@chu-nantes.fr
Site Name
Centre Hospitalier General De St Denis
Department Name
Neurology
Principal Investigator Name
Carole HENRY
Principal Investigator Email
carole.henry@ch-stdenis.fr
Contact Person Name
Carole HENRY
Contact Person Email
carole.henry@ch-stdenis.fr
Site Name
Centre Hospitalier Sud Francilien
Department Name
Neurology
Principal Investigator Name
Nicolas CHAUSSON
Principal Investigator Email
nicolas.chausson@chsf.fr
Contact Person Name
Nicolas CHAUSSON
Contact Person Email
nicolas.chausson@chsf.fr
Site Name
Centre Hospitalier De Perpignan
Department Name
Neurology
Principal Investigator Name
DENIS SABLOT
Principal Investigator Email
denis.sablot@ch-perpignan.fr
Contact Person Name
DENIS SABLOT
Contact Person Email
denis.sablot@ch-perpignan.fr
Site Name
Hospital Foch
Department Name
Neurology
Principal Investigator Name
Bertrand LAPERGUE
Principal Investigator Email
b.lapergue@hopital-foch.com
Contact Person Name
Bertrand LAPERGUE
Contact Person Email
b.lapergue@hopital-foch.com
Site Name
Centre Hospitalier Regional De Marseille
Department Name
Neurology
Principal Investigator Name
Laurent SUISSA
Principal Investigator Email
laurent.suissa@ap-hm.fr
Contact Person Name
Laurent SUISSA
Contact Person Email
laurent.suissa@ap-hm.fr
Site Name
Fondation A De Rothschild
Department Name
Neurology
Principal Investigator Name
Michael OBADIA
Principal Investigator Email
mobadia@for.paris
Contact Person Name
Michael OBADIA
Contact Person Email
mobadia@for.paris
Site Name
Les Hopitaux Universitaires De Strasbourg
Department Name
Neurology
Principal Investigator Name
Valérie WOLFF
Principal Investigator Email
valerie.wolff@chru-strasbourg.fr
Contact Person Name
Valérie WOLFF
Site Name
Centre Hospitalier Universitaire De Nice
Department Name
Neurology
Principal Investigator Name
Barbara CASOLLA
Principal Investigator Email
casolla.b@chu-nice.fr
Contact Person Name
Barbara CASOLLA
Contact Person Email
casolla.b@chu-nice.fr
Site Name
Hopitaux Universitaires Pitie Salpetriere
Department Name
Neurology
Principal Investigator Name
Gaspard GERSCHENFELD
Principal Investigator Email
gaspard.gerschenfeld@aphp.fr
Contact Person Name
Gaspard GERSCHENFELD
Contact Person Email
gaspard.gerschenfeld@aphp.fr
Site Name
Centre Hospitalier Du Pays D Aix Centre Hospitalier Intercommunal Aix-Pertuis
Department Name
Neurology
Principal Investigator Name
Silvia DI LEGGE
Principal Investigator Email
sdilegge@ch-aix.fr
Contact Person Name
Silvia DI LEGGE
Contact Person Email
sdilegge@ch-aix.fr
Site Name
Centre Hospitalier Universitaire De Rennes
Department Name
Neurology
Principal Investigator Name
Stéphane VANNIER
Principal Investigator Email
stephane.vannier@chu-rennes.fr
Contact Person Name
Stéphane VANNIER
Contact Person Email
stephane.vannier@chu-rennes.fr
Site Name
Assistance Publique Hopitaux De Paris (46 Rue Henri Huchard)
Department Name
Neurology
Principal Investigator Name
Philippa LAVALLEE
Principal Investigator Email
philippa.lavallee@aphp.fr
Contact Person Name
Philippa LAVALLEE
Contact Person Email
philippa.lavallee@aphp.fr
Site Name
Bicetre Hospital
Department Name
Neurology
Principal Investigator Name
Laura VENDITTI
Principal Investigator Email
laura.venditti@aphp.fr
Contact Person Name
Laura VENDITTI
Contact Person Email
laura.venditti@aphp.fr
Site Name
GIE Groupe hospitalier Paris Saint-Joseph/Vinci
Department Name
Neurology
Principal Investigator Name
Benjamin MAIER
Principal Investigator Email
bmaier@ghpsj.fr
Contact Person Name
Benjamin MAIER
Contact Person Email
bmaier@ghpsj.fr
Site Name
Centre Hospitalier De Versailles
Department Name
Neurology
Principal Investigator Name
Fernando PICO
Principal Investigator Email
fpico@ght78sud.fr
Contact Person Name
Fernando PICO
Contact Person Email
fpico@ght78sud.fr
Site Name
Assistance Publique Hopitaux De Paris (2 Rue Ambroise Pare)
Department Name
Neurology
Principal Investigator Name
Elodie BERTHET
Principal Investigator Email
elodie.berthet@aphp.fr
Contact Person Name
Elodie BERTHET
Contact Person Email
elodie.berthet@aphp.fr
Site Name
Centre Hospitalier Universitaire De Caen Normandie
Department Name
Neurology
Principal Investigator Name
Marion BOULANGER
Principal Investigator Email
boulanger-ma@chu-caen.fr
Contact Person Name
Marion BOULANGER
Contact Person Email
boulanger-ma@chu-caen.fr
Site Name
Centre Hospitalier Universitaire De Lille
Department Name
Neurology
Principal Investigator Name
Lucie DELLA SCHIAVA
Principal Investigator Email
lucie.dellaschiava@chu-llile.fr
Contact Person Name
Lucie DELLA SCHIAVA
Site Name
Hospices Civils De Lyon
Department Name
Neurology
Principal Investigator Name
Tae-Hee CHO
Principal Investigator Email
tae-hee.cho@chu-lyon
Contact Person Name
Tae-Hee CHO
Contact Person Email
tae-hee.cho@chu-lyon
Site Name
CHRU De Nancy
Department Name
Neurology
Principal Investigator Name
Sébastien RICHARD
Principal Investigator Email
s.richard@chru-nancy.fr
Contact Person Name
Sébastien RICHARD
Contact Person Email
s.richard@chru-nancy.fr
Site Name
Centre Hospitalier Et Universitaire De Limoges
Department Name
Neurology
Principal Investigator Name
Francisco MACIAN-MONTORO
Principal Investigator Email
francisco.macianmontoro@chu-limoges.fr
Contact Person Name
Francisco MACIAN-MONTORO
Site Name
Centre Hospitalier Universitaire De Saint Etienne
Department Name
Neurology
Principal Investigator Name
Pierre GARNIER
Principal Investigator Email
pierre.garnier@chu-st-etienne.fr
Contact Person Name
Pierre GARNIER
Site Name
Centre Hospitalier Regional Universitaire De Tours
Department Name
Neurology
Principal Investigator Name
Marco PASI
Principal Investigator Email
m.pasi@chu-tours.fr
Contact Person Name
Marco PASI
Contact Person Email
m.pasi@chu-tours.fr
Site Name
Centre Hospitalier Universitaire Rouen
Department Name
Neurology
Principal Investigator Name
Florient BASILE
Principal Investigator Email
florian.basille@chu-rouen.fr
Contact Person Name
Florient BASILE
Contact Person Email
florian.basille@chu-rouen.fr
Site Name
Centre Hospitalier Universitaire De Poitiers
Department Name
Neurology
Principal Investigator Name
Nathalie NASR
Principal Investigator Email
nathalie.nasr@chu-poitiers.fr
Contact Person Name
Nathalie NASR
Contact Person Email
nathalie.nasr@chu-poitiers.fr
Site Name
Centre Hospitalier Universitaire De Dijon
Department Name
Neurology
Principal Investigator Name
Yannick BEJOT
Principal Investigator Email
yannick.bejot@chu-dijon.fr
Contact Person Name
Yannick BEJOT
Contact Person Email
yannick.bejot@chu-dijon.fr
Site Name
Assistance Publique Hopitaux De Paris (51 Av Du Mal De Lattre De Tassigny)
Department Name
Neurology
Principal Investigator Name
Aymeric WITTWER
Principal Investigator Email
aymeric.wittwer@aphp.fr
Contact Person Name
Aymeric WITTWER
Contact Person Email
aymeric.wittwer@aphp.fr
Site Name
Centre Hospitalier Universitaire Reims
Department Name
Neurology
Principal Investigator Name
Solène MOULIN
Principal Investigator Email
smoulin@chu-reims.fr
Contact Person Name
Solène MOULIN
Contact Person Email
smoulin@chu-reims.fr
Site Name
CHU Besancon
Department Name
Neurology
Principal Investigator Name
Guillaume CHARBONNIER
Principal Investigator Email
guillaume.charbonnier@univ-fcomte.fr
Contact Person Name
Guillaume CHARBONNIER
Site Name
Centre Hospitalier General
Department Name
Neurology
Principal Investigator Name
Eric MANCHON
Principal Investigator Email
eric.manchon@ch-gonesse.fr
Contact Person Name
Eric MANCHON
Contact Person Email
eric.manchon@ch-gonesse.fr
Site Name
Groupe Hospitalier Universitaire Paris Psychiatrie Et Neuroscience
Department Name
Neurology
Principal Investigator Name
Guillaume TURC
Principal Investigator Email
g.turc@ghu-paris.fr
Contact Person Name
Guillaume TURC
Contact Person Email
g.turc@ghu-paris.fr
Site Name
Centre Hospitalier Universitaire De Bordeaux
Department Name
Neurology
Principal Investigator Name
Igor SIBON
Principal Investigator Email
igor.sibon@chu-bordeaux.fr
Contact Person Name
Igor SIBON
Contact Person Email
igor.sibon@chu-bordeaux.fr

Sponsor

Primary sponsor

Full Name
Assistance Publique Hopitaux De Paris
Organisation Type
Hospital/Clinic/Other health care facility
Country Of Registered Address
France

Investigational products

Investigational Product Name
Metalyse 5 000 units (25 mg) powder for solution for injection
Active Substance
TENECTEPLASE
Modality
Peptide/protein/enzyme
Routes Of Administration
INTRAVENOUS BOLUS USE
Route
Intravenous bolus
Authorisation Status
Authorised (marketing authorisation EU/1/00/169/007)
Starting Dose
25 mg
Dose Levels
25 mg
Frequency
Single administration (per protocol for IV thrombolysis)
Maximum Dose
25 mg
Combination Treatment
Yes

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